产品介绍
基本信息
| 规格 | 50ug |
|---|---|
| 表达宿主 | E.coli |
| 标签 | NA |
物理性质
| 分子量 | 13 KDa |
|---|---|
| 表观分子量 | 14 KDa, reducing conditions |
| 形态 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2. |
| 纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
活性与内毒素
| 内毒素水平 | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
|---|---|
| 活性 | Immobilized Human TrkB-His (Cat#C507) at 2ug/ml (100 μl/well) can bind Human BDNF*(Cat#C076)*: Biotinylated by NHS-biotin prior to testing. The ED50 of Human BDNF*(Cat#C076) is 47.58 ng/ml. |
储存与运输
| 溶解方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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| 储存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
详细说明
| 背景说明 | Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. Along with other structurally related neurotrophic factors NGF, NT-3 and NT-4, BDNF binds with high affinity to the TrkB kinase receptor. It also binds with the LNGFR (for low-affinity nerve growth factor receptor, also known as p75). BDNF promotes the survival, growth and differentiation of neurons. It serves as a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. BDNF expression is altered in neurodegenerative disorders such as Parkinson's and Alzheimer's disease. |
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