产品介绍

基本信息

规格	50ug
表达宿主	Human Cells
标签	N-6His

物理性质

分子量	31.5 KDa
表观分子量	30-40 KDa, reducing conditions
形态	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
纯度	Greater than 95% as determined by reducing SDS-PAGE.

活性与内毒素

内毒素水平	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

储存与运输

溶解方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
储存方法	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
运输条件	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

详细说明

背景说明

Langerin (CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family. Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis. Langerin is necessary and sufficient for Birbeck granule formation. Human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Human langerin shares 68%, 62%, 71% aa identity with mouse, rat and bovine langerin ECD, respectively. Trimerization greatly increases the lectin binding affinity. Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions. Langerin also mediates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae.