产品介绍
基本信息
| 规格 | 10ug |
|---|---|
| 表达宿主 | E.coli |
| 标签 | NA |
物理性质
| 分子量 | 12.42 KDa |
|---|---|
| 表观分子量 | 14 KDa, reducing conditions |
| 形态 | Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, pH 4.5. |
| 纯度 | Greater than 98% as determined by reducing SDS-PAGE. |
活性与内毒素
| 内毒素水平 | Less than 0.02 ng/µg (0.2 EU/µg) as determined by LAL test. |
|---|---|
| 活性 | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 5-20 ng/ml. |
储存与运输
| 溶解方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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| 储存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
详细说明
| 背景说明 | Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. |
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