产品介绍

基本信息

规格	50ug
表达宿主	Human Cells
标签	C-6His

物理性质

分子量	47.3 KDa
表观分子量	65-90 KDa, reducing conditions
形态	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
纯度	Greater than 95% as determined by reducing SDS-PAGE.

活性与内毒素

内毒素水平	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
活性	Immobilized Human B7-H3-His(Cat#CK62) at 2μg/ml (100 μl/well) can bind Anti-Human B7-H3 mAb(Cat#NC055 ) The ED50 of Anti-Human B7-H3 mAb(Cat#NC055 ) is 7.98 ng/ml.

储存与运输

溶解方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
储存方法	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
运输条件	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

详细说明

背景说明

CD276, also known as B7-H3, is a member of the B7 superfamily with signature IgV and IgG regions in extracellular domains. It is a type I transmembrane protein and shares 20–27% amino acid identity with other B7 family members. B7-H3 is involved in the activation of T lymphocytes, and regulates murine bone formation. It is also reported that B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is expressed on T-cells, natural killer cells, and antigen presenting cells, as well as some non-immune cells, such as osteoblasts, fibroblasts, fibroblast-like synoviocytes and epithelial cells. High expression of B7-H3 in tumor vasculature also correlates with poor survival in patients, suggesting that it may play a role in tumor cell migration.