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Recombinant Human EGF

  • 货号:EPT004
  • 价格:¥240.00 元
  • 点击:1456
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产品介绍

基本信息

规格10ug
表达宿主E.coli
标签NA

物理性质

分子量6.2 KDa
表观分子量11 KDa, reducing conditions
形态Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 200mM NaCl, pH 8.0.
纯度Greater than 95% as determined by reducing SDS-PAGE.

活性与内毒素

内毒素水平Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
活性Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml.

储存与运输

溶解方法Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
储存方法Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
运输条件The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.

详细说明

背景说明Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.
【仅供科学研究使用】

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