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小鼠WNT1诱导信号通道蛋白2(WISP2)酶联免疫吸附检测试剂盒

  • 货号:ELK0044
  • 价格:¥2,600.00 元
  • 点击:1113

产品介绍

产品信息

英文名称Mouse WISP2(WNT1 Inducible Signaling Pathway Protein 2) ELISA Kit
别名CCN5; CT58; CTGF-L; CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Mouse
检测类型Sandwich
灵敏度0.069 ng/mL
检测范围0.16-10 ng/mL
样本类型Tissue homogenates, cell lysates and other biological fluids
实验时长3.5h

研究信息

研究领域Signal transduction;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse WISP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse WISP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse WISP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse WISP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q9Z0G4

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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