{"48t":"1960","96t":"2800","96t_5":"11900","name_en":"EasyStep Human OC(Osteocalcin) ELISA Kit","alternative_names":"BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein","species":"Human","assay_type":"Sandwich","sensitivity":"0.14 ng\/mL","standard":"15 ng\/mL","detection_range":"0.23-15 ng\/mL","sample_type":"Serum","assay_length":"1.5h","research_area":"Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OC, and the Human OC standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human OC. After TMB substrate solution is added, only those wells that contain Human OC and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OC in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P02818","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK009ES-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK009ES-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK009ES",}