人碳酸酐酶XI(CA11)酶联免疫吸附检测试剂盒

英文名称	Human CA11(Carbonic Anhydrase 11) ELISA Kit
别名	Carbonic anhydrase-related protein 11; CAH11; CA-RP XI; CA-XI; CARP XI; Carbonic anhydrase-related protein 2; CA-RP II; CARP-2

种属	Human
检测类型	Sandwich
灵敏度	0.089 ng/mL
检测范围	0.32-20 ng/mL
样本类型	Serum, plasma, saliva and other biological fluids
实验时长	3.5h

研究领域	Enzyme & Kinase;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA11 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	O75493

标准曲线图

【仅供科学研究使用】

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