8异前列腺素F2α(8-iso-PGF2a)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"8-iso-PGF2α(8-isoprostane) ELISA Kit","alternative_names":"8-isoprostane; 8-iso-PGF2α","species":"General","assay_type":"Competitive Inhibition","sensitivity":"4.47 pg\/mL","standard":"1000 pg\/mL","detection_range":"15.63-1000 pg\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"2.5h","research_area":"Endocrinology;Pulmonology;Hepatology;Hormone metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-iso-PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-iso-PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-iso-PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0151-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0151-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0151",}