大鼠糖抗原19-9(CA19-9)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Rat CA19-9(Carbohydrate Antigen 19-9) Microsample ELISA Kit","alternative_names":"sialylated Lewis(a)Antigen; Cancer Antigen 19-9","species":"Rat","assay_type":"Sandwich","sensitivity":"0.3 U\/mL","standard":"50 U\/mL","detection_range":"0.79-50 U\/mL","sample_type":"Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.","assay_length":"3.5h","research_area":"Tumor immunity;Hepatology;Gastroenterology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0162MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0162MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0162MS",}