大鼠激活素A(ACV-A)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Rat ACV-A(Activin A) ELISA Kit","alternative_names":"ACVA; Activin Beta A Beta A Homodimer","species":"Rat","assay_type":"Sandwich","sensitivity":"4.57 pg\/mL","standard":"1000 pg\/mL","detection_range":"15.63-1000 pg\/mL","sample_type":"Serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Endocrinology;Reproductive science;Hormone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACV-A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ACV-A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ACV-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ACV-A in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0175-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0175-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0175",}