一步法大鼠雌二醇(E2)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"EasyStep Rat E2(Estradiol) ELISA Kit","alternative_names":"17B-Estradiol; Oestradiol; Beta-Estradiol","species":"Rat","assay_type":"Competitive Inhibition","sensitivity":"25 pg\/mL","standard":"1600 pg\/mL","detection_range":"25-1600 pg\/mL","sample_type":"Serum","assay_length":"1.5h","research_area":"Endocrinology;Reproductive science;Hormone metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat E2 antigen, and the Rat E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat E2 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK022ES-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK022ES-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK022ES",}