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人细胞色素P450家族成员3A5(CYP3A5)酶联免疫吸附检测试剂盒

  • 货号:ELK0270
  • 价格:¥2,800.00 元
  • 点击:1782

产品介绍

产品信息

英文名称Human CYP3A5(Cytochrome P450 3A5) ELISA Kit
别名CP35; P450PCN3; PCN3; Cytochrome P450,Subfamily IIIA(Niphedipine Oxidase),Polypeptide 5; Cytochrome P450 HLp2

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Human
检测类型Sandwich
灵敏度0.047 ng/mL
检测范围0.16-10 ng/mL
样本类型serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Tumor immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYP3A5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYP3A5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYP3A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYP3A5 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号CYP3A5

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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