人真核起始因子4E2(EIF4E2)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Human EIF4E2(Eukaryotic Translation Initiation Factor 4E Type 2) ELISA Kit","alternative_names":"eIF-4E type 2; eIF4E type 2; Eukaryotic translation initiation factor 4E homologous protein; Eukaryotic translation initiation factor 4E-like 3; eIF4E-like protein 4E-LP; mRNA cap-binding protein 4EHP; h4EHP; mRNA cap-binding protein type 3","species":"Human","assay_type":"Sandwich","sensitivity":"11.9 pg\/mL","standard":"2000 pg\/mL","detection_range":"31.25-2000 pg\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Breast Cancer","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EIF4E2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EIF4E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EIF4E2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EIF4E2 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"O60573","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0281-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0281-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0281",}