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一步法人血管内皮生长因子A(VEGFA)酶联免疫吸附检测试剂盒

  • 货号:ELK033ES
  • 价格:¥2,400.00 元
  • 点击:1298

产品介绍

产品信息

英文名称EasyStep Human VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit
别名VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor

规格与价格

48T 价格1680
96T 价格2400
96T*5 价格10200

检测参数

种属Human
检测类型Sandwich
灵敏度31 pg/mL
检测范围78.2-5000 pg/mL
样本类型Serum, plasma
实验时长1.5h

研究信息

研究领域Cytokine;Apoptosis;Tumor immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFA, and the Human VEGFA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGFA. After TMB substrate solution is added, only those wells that contain Human VEGFA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P15692

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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