一步法人血管内皮生长因子A(VEGFA)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"EasyStep Human VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit","alternative_names":"VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor","species":"Human","assay_type":"Sandwich","sensitivity":"31 pg\/mL","standard":"5000 pg\/mL","detection_range":"78.2-5000 pg\/mL","sample_type":"Serum, plasma","assay_length":"1.5h","research_area":"Cytokine;Apoptosis;Tumor immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFA, and the Human VEGFA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGFA. After TMB substrate solution is added, only those wells that contain Human VEGFA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P15692","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK033ES-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK033ES-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK033ES",}