一步法人骨保护素(OPG)酶联免疫吸附检测试剂盒

英文名称	EasyStep Human OPG(Osteoprotegerin) ELISA Kit
别名	TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor

研究领域	Cytokine;Metabolic pathway;Bone metabolism;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OPG, and the Human OPG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human OPG. After TMB substrate solution is added, only those wells that contain Human OPG and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OPG in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	O00300

标准曲线图

【仅供科学研究使用】

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