一步法人氨基端前脑钠素(NT-ProBNP)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"EasyStep Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit","alternative_names":"NT-Pro-BNP; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb","species":"Human","assay_type":"Sandwich","sensitivity":"0.94 ng\/mL","standard":"60 ng\/mL","detection_range":"0.94-60 ng\/mL","sample_type":"Serum, plasma","assay_length":"1.5h","research_area":"Endocrinology;Cardiovascular biology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP, and the Human NT-ProBNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NT-ProBNP. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK035ES-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK035ES-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK035ES",}