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小鼠转录激活因子4(ATF4)酶联免疫吸附检测试剂盒

  • 货号:ELK0365
  • 价格:¥2,800.00 元
  • 点击:2628

产品介绍

产品信息

英文名称Mouse ATF4(Activating Transcription Factor 4) ELISA Kit
别名TAXREB67; TXREB; CREB-2; DNA-binding protein TAXREB67; Cyclic AMP-responsive element-binding protein 2; Tax-responsive enhancer element-binding protein 67

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Mouse
检测类型Sandwich
灵敏度30.4 pg/mL
检测范围78.13-5000 pg/mL
样本类型Tissue homogenates, cell lysates and other biological fluids.
实验时长3.5h

研究信息

研究领域Signal transduction;Developmental science;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ATF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ATF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ATF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ATF4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q06507

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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