人可溶性清道夫受体富含半胱氨酸结构域蛋白SSC5D(SSC5D)酶联免疫吸附检测试剂盒

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• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human SSC5D(Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D) ELISA Kit","alternative_names":"SRCRL; Soluble scavenger protein with 5 SRCR domains; SSc5D","species":"Human","assay_type":"Sandwich","sensitivity":"0.14 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SSC5D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SSC5D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SSC5D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SSC5D in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0401-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0401-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0401",}