一步法大鼠游离三碘甲状腺原氨酸(T3)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit","alternative_names":"3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin","species":"Rat","assay_type":"Competitive Inhibition","sensitivity":"0.31 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"Serum, plasma","assay_length":"1.5h","research_area":"Endocrinology;Autoimmunity;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat fT3 antigen, and the Rat fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK050ES-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK050ES-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK050ES",}