一步法人25-羟基维生素D(25-OH-D)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"EasyStep Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit","alternative_names":"25 Hydroxy Vitamin D;","species":"Human","assay_type":"Competitive Inhibition","sensitivity":"7.7 ng\/mL","standard":"5000 ng\/mL","detection_range":"78.13-5000 ng\/mL","sample_type":"Serum, plasma","assay_length":"1.5h","research_area":"Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D antigen, and the Human 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK051ES-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK051ES-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK051ES",}