大鼠乙酰辅酶A乙酰转移酶1(ACAT1)酶联免疫吸附检测试剂盒

英文名称	Rat ACAT1(Acetyl Coenzyme A Acetyltransferase 1) ELISA Kit
别名	THIL; ACAT; T2; MAT; Acetoacetyl Coenzyme A Thiolase

种属	Rat
检测类型	Sandwich
灵敏度	16.6 pg/mL
检测范围	31.25-2000 pg/mL
样本类型	Tissue homogenates, cell lysates and other biological fluids
实验时长	3.5h

研究领域	Enzyme & Kinase;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ACAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ACAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ACAT1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P17764

标准曲线图

【仅供科学研究使用】

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