斑马鱼γ-氨基丁酸(gABA)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Zebrafish gABA(Gamma-Aminobutyric Acid) ELISA Kit","alternative_names":"4-Aminobutyric Acid","species":"Zebrafish","assay_type":"Competitive Inhibition","sensitivity":"16.7 pg\/mL","standard":"2000 pg\/mL","detection_range":"31.25-2000 pg\/mL","sample_type":"serum, plasma, cell lysates, cell culture supernates and other biological fluids","assay_length":"2.5h","research_area":"Signal transduction;Cardiovascular biology;Neuro science;Bone metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish gABA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish gABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish gABA in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0580-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0580-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0580",}