一步法大鼠胰岛素样生长因子结合蛋白7(IGFBP7)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"EasyStep Rat IGFBP7(Insulin Like Growth Factor Binding Protein 7) ELISA Kit","alternative_names":"FSTL2; IGFBP-7v; MAC25; PSF; TAF; IGFBP-rP1; MAC25 protein; Prostacyclin-stimulating factor; PGI2-stimulating factor; Tumor-derived adhesion factor","species":"Rat","assay_type":"Sandwich","sensitivity":"7 ng\/mL","standard":"1000 ng\/mL","detection_range":"15.63-1000 ng\/mL","sample_type":"Serum, plasma","assay_length":"1.5h","research_area":"Metabolic pathway;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP7, and the Rat IGFBP7 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat IGFBP7. After TMB substrate solution is added, only those wells that contain Rat IGFBP7 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IGFBP7 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK064ES-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK064ES-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK064ES",}