一步法人干扰素γ(IFNg)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"EasyStep Human IFNg(Interferon Gamma) ELISA Kit","alternative_names":"IFN gamma; IFN-G; IFG; IFI; INFr; IFN; Immune Interferon","species":"Human","assay_type":"Sandwich","sensitivity":"15 pg\/mL","standard":"1000 pg\/mL","detection_range":"15.63-1000 pg\/mL","sample_type":"Serum, plasma, cell","assay_length":"1.5h","research_area":"Cytokine;Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ, and the Human IFN-γ standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IFN-γ. After TMB substrate solution is added, only those wells that contain Human IFN-γ and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFN-γ in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK067ES-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK067ES-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK067ES",}