人Rho相关蛋白激酶1(Rock-1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human Rock1(Rho-associated protein kinase 1) ELISA Kit","alternative_names":"P160ROCK; ROCK-I","species":"Human","assay_type":"Sandwich","sensitivity":"0.15 ng\/mL","standard":"20 ng\/ml","detection_range":"0.32-20 ng\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Rock-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Rock-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Rock-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Rock-1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q13464","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0699-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0699-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0699",}