大鼠T细胞表面糖蛋白CD1A(CD1A)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Rat CD1A (T-Cell Surface Glycoprotein CD1A) ELISA Kit","alternative_names":"CD1-A; CD1; FCB6; HTA1; R4; T6; T-cell surface antigen T6\/Leu-6; hTa1 thymocyte antigen","species":"Rat","assay_type":"Sandwich","sensitivity":"0.07 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CD1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CD1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CD1A in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0716-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0716-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0716",}