{"48t":"1960","96t":"2800","96t_5":"11900","name_en":"EasyStep Human TF(Tissue Factor) ELISA Kit","alternative_names":"CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III","species":"Human","assay_type":"Sandwich","sensitivity":"0.12 pg\/mL","standard":"800 pg\/mL","detection_range":"12.5-800 pg\/mL","sample_type":"Serum, plasma","assay_length":"1.5h","research_area":"Hematology;Cardiovascular biology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TF, and the Human TF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human TF. After TMB substrate solution is added, only those wells that contain Human TF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TF in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK073ES-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK073ES-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK073ES",}