{"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Human G-17(Gastrin-17) ELISA Kit","alternative_names":"Gastrin17; Gas17","species":"Human","assay_type":"Sandwich","sensitivity":"14.3 pg\/mL","standard":"2000 pg\/mL","detection_range":"31.25-2000 pg\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human G-17. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human G-17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human G-17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human G-17 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0740-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0740-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0740",}