人抗组织谷氨酰胺转移酶抗体IgA(Anti-tTG-IgA)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit","alternative_names":"Anti-Tissue Transglutaminase IgA","species":"Human","assay_type":"Sandwich","sensitivity":"1.45 IU\/mL","standard":"300 IU\/mL","detection_range":"4.69-300 IU\/mL","sample_type":"Serum, plasma, tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG-IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG-IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG-IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG-IgA in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0752-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0752-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0752",}