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首页>产品中心>Elisa试剂盒>小鼠磷酸化胰岛素受体β亚单位IR (Phospho) [pY1162/1163]酶联免疫吸附检测试剂盒
小鼠磷酸化胰岛素受体β亚单位IR (Phospho) [pY1162/1163]酶联免疫吸附检测试剂盒
- 货号：ELK0766
- 价格：￥2,800.00 元
- 点击：649
产品介绍
产品信息
| 英文名称 | Mouse phospho-Insulin Receptor, β Subunit(pTyr1162/1163) ELISA Kit |
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| 别名 | p-IR |
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规格与价格
| 48T 价格 | 1960 |
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| 96T 价格 | 2800 |
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| 96T*5 价格 | 11900 |
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检测参数
| 种属 | Mouse |
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| 检测类型 | Sandwich |
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| 灵敏度 | 0.68 U/mL |
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| 检测范围 | 1.57-100 U/mL |
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| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| 实验时长 | 3.5h |
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研究信息
| 研究领域 | Signal transduction;Endocrinology;Autoimmunity; |
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| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IR (Phospho) [pY1162/1163]. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IR (Phospho) [pY1162/1163]. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IR (Phospho) [pY1162/1163], biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IR (Phospho) [pY1162/1163] in the samples is then determined by comparing the OD of the samples to the standard curve. |
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标准曲线
| 标准曲线图 |  |
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