人2,3-二磷酸甘油酸(2,3-DPG)酶联免疫吸附检测试剂盒
- 货号：ELK0778
- 价格：￥2,800.00 元
- 点击：1897
产品介绍
产品信息
| 英文名称 | Human 2,3-DPG(2,3-Diphosphoglycerate) ELISA Kit |
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| 别名 | 2,3DPG |
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规格与价格
| 48T 价格 | 1960 |
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| 96T 价格 | 2800 |
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| 96T*5 价格 | 11900 |
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检测参数
| 种属 | Human |
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| 检测类型 | Sandwich |
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| 灵敏度 | 0.14 µmol/mL |
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| 检测范围 | 0.32-20 µmol/mL |
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| 样本类型 | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
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| 实验时长 | 3.5h |
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研究信息
| 研究领域 | Metabolic pathway |
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| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 2,3-DPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 2,3-DPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 2,3-DPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 2,3-DPG in the samples is then determined by comparing the OD of the samples to the standard curve. |
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标准曲线
| 标准曲线图 |  |
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