人2,3-二磷酸甘油酸(2,3-DPG)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Human 2,3-DPG(2,3-Diphosphoglycerate) ELISA Kit","alternative_names":"2,3DPG","species":"Human","assay_type":"Sandwich","sensitivity":"0.14 µmol\/mL","standard":"20 µmol\/mL","detection_range":"0.32-20 µmol\/mL","sample_type":"Serum, plasma, tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Metabolic pathway","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 2,3-DPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 2,3-DPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 2,3-DPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 2,3-DPG in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0778-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0778-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0778",}