大鼠电碳酸氢钠协同转运蛋白4(NBC4)酶联免疫吸附检测试剂盒

英文名称	Rat NBC4(Electrogenic Sodium Bicarbonate Cotransporter 4) ELISA Kit
别名	SLC4A5; NBCe2; Solute Carrier Family 4 Member 5,Sodium Bicarbonate Cotransporter

种属	Rat
检测类型	Sandwich
灵敏度	0.32 ng/mL
检测范围	0.79-50 ng/mL
样本类型	Tissue homogenates and other biological fluids.
实验时长	3.5h

研究领域	Signal transduction;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NBC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NBC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NBC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NBC4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q6RI88

标准曲线图

【仅供科学研究使用】

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