一步法大鼠血管内皮生长因子受体2(VEGFR2)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"EasyStep Rat VEGFR2(Vascular Endothelial Growth Factor Receptor 2) ELISA Kit","alternative_names":"CD309; FLK1; VEGFR; KDR; A Type III Receptor Tyrosine Kinase; Kinase Insert Domain Receptor; Kinase Insert Domain Receptor; Fetal Liver Kinase-1","species":"Rat","assay_type":"Sandwich","sensitivity":"157.5 pg\/mL","standard":"50 ng\/mL","detection_range":"0.78-50 ng\/mL","sample_type":"Serum, plasma","assay_length":"1.5h","research_area":"Signal transduction;CD & Adhesion molecule;Tumor immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VEGFR2, and the Rat VEGFR2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat VEGFR2. After TMB substrate solution is added, only those wells that contain Rat VEGFR2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VEGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK083ES-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK083ES-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK083ES",}