{"48t":"1680","96t":"2400","96t_5":"10200","name_en":"EasyStep Human IL8(Interleukin 8) ELISA Kit","alternative_names":"IL-8; CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1","species":"Human","assay_type":"Sandwich","sensitivity":"3.44 pg\/mL","standard":"400 pg\/mL","detection_range":"6.25-400 pg\/mL","sample_type":"Cell culture supernatants","assay_length":"1.5h","research_area":"Cytokine;Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL8, and the Human IL8 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL8. After TMB substrate solution is added, only those wells that contain Human IL8 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL8 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK085ES-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK085ES-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK085ES",}