小鼠肽基精氨酸脱亚氨酶Ⅵ(PADI6)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Mouse PADI6(Peptidyl Arginine Deiminase Type VI) ELISA Kit","alternative_names":"Peptidylarginine deiminase VI; Peptidyl arginine deiminase-like protein","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.073 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"Enzyme & Kinase;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PADI6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PADI6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PADI6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PADI6 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q8K3V4","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0886-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0886-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0886",}