猪血管内皮生长因子C(VEGFC)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Pig VEGFC(Vascular Endothelial Growth Factor C) ELISA Kit","alternative_names":"VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein","species":"Pig","assay_type":"Sandwich","sensitivity":"25.8 pg\/mL","standard":"4000 pg\/mL","detection_range":"62.5-4000 pg\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Cytokine;Tumor immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig VEGFC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig VEGFC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig VEGFC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig VEGFC in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0896-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0896-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0896",}