一步法人甲胎蛋白(aFP)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"EasyStep Human aFP(Alpha-Fetoprotein) ELISA Kit","alternative_names":"A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein","species":"Human","assay_type":"Sandwich","sensitivity":"0.175 ng\/mL","standard":"100 ng\/mL","detection_range":"1.56-100 ng\/mL","sample_type":"Serum, plasma","assay_length":"1.5h","research_area":"Tumor immunity;Hepatology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human aFP, and the Human aFP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human aFP. After TMB substrate solution is added, only those wells that contain Human aFP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aFP in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK091ES-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK091ES-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK091ES",}