大鼠磷酸化c-Jun氨基末端激酶(p-JNK)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat p-JNK(Phosphorylated c-Jun N-terminal kinases) ELISA Kit","alternative_names":"Bsk; BSK; CG5680; D-JNK; D-junk; DBSK\/JNK; dJnk; dJNK; DJNK; DJNK\/bsk; Dmel\\CG5680; DmJNK; dNJNK; jnk; Jnk; JNK; jnk (bsk); JNK\/SAPK; Junk; p-JNK; P-JNK; pJNK; SAPKa","species":"Rat","assay_type":"Sandwich","sensitivity":"0.148 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-JNK in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0960-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0960-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0960",}