人淋巴细胞趋化因子(Lptn/LTN/XCL1)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Human Lptn\/LTN\/XCL1 (Lymphotactin) Microsample ELISA Kit","alternative_names":"Xcl1 ; Lptn ; Ltn ; Scyc1 ; Lymphotactin ; C motif chemokine 1 ; Cytokine SCM-1 ; Lymphotaxin ; Small-inducible cytokine C1","species":"Human","assay_type":"Sandwich","sensitivity":"0.12 ng\/mL","standard":"25 ng\/mL","detection_range":"0.39-25 ng\/mL","sample_type":"Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.","assay_length":"3.5h","research_area":"Cytokine;Tumor immunity;Infection immunity;Dermatology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Lptn\/LTN\/XCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Lptn\/LTN\/XCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Lptn\/LTN\/XCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Lptn\/LTN\/XCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P47992","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10035MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10035MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10035MS",}