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人垂体同源盒蛋白1(PITX1)酶联免疫吸附检测试剂盒

  • 货号:ELK10073
  • 价格:¥2,400.00 元
  • 点击:1720

产品介绍

产品信息

英文名称Human PITX1(Pituitary homeobox 1) ELISA Kit
别名Pituitary homeobox 1;PITX1;Hindlimb-expressed homeobox protein backfoot;Homeobox protein PITX1;Paired-like homeodomain transcription factor 1;BFT,;PTX1

规格与价格

48T 价格1680
96T 价格2400
96T*5 价格10200

检测参数

种属Human
检测类型Sandwich
灵敏度0.095 ng/mL
检测范围0.16-10 ng/mL
样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长3.5h

研究信息

研究领域Immunology;Cancer
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PITX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PITX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PITX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PITX1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P78337

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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