人垂体同源盒蛋白1(PITX1)酶联免疫吸附检测试剂盒

英文名称	Human PITX1(Pituitary homeobox 1) ELISA Kit
别名	Pituitary homeobox 1;PITX1;Hindlimb-expressed homeobox protein backfoot;Homeobox protein PITX1;Paired-like homeodomain transcription factor 1;BFT,;PTX1

种属	Human
检测类型	Sandwich
灵敏度	0.095 ng/mL
检测范围	0.16-10 ng/mL
样本类型	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长	3.5h

研究领域	Immunology;Cancer
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PITX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PITX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PITX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PITX1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P78337

标准曲线图

【仅供科学研究使用】

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