小鼠肿瘤坏死因子α诱导蛋白3(TNFaIP3)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Mouse TNFaIP3(Tumor Necrosis Factor Alpha Induced Protein 3) ELISA Kit","alternative_names":"A20; TNF alpha-induced protein 3; Putative DNA-binding protein A20; Zinc finger protein A20","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.093 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.","assay_length":"3.5h","research_area":"Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFaIP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TNFaIP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TNFaIP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TNFaIP3 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q60769","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10090-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10090-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10090",}