小鼠胱抑素C(Cys-C)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Mouse Cys-C(Cystatin C) ELISA Kit","alternative_names":"CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin; Gamma Trace; Post-Gamma-Globulin; Neuroendocrine Basic Polypeptide; Amyloid Angiopathy And Cerebral Hemorrhage","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.49 ng\/mL","standard":"100 ng\/mL","detection_range":"1.57-100 ng\/mL","sample_type":"Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.","assay_length":"3.5h","research_area":"Kidney biomarker;Urology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P21460","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10181-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10181-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10181",}