大鼠高敏C反应蛋白(hs-CRP)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Rat hs-CRP(High Sensitivity C Reactive Protein) Microsample ELISA Kit","alternative_names":"C-RP; PTX1; Pentraxin-Related; Pentraxin 1","species":"Rat","assay_type":"Sandwich","sensitivity":"1.08 µg\/mL","standard":"100 µg\/mL","detection_range":"1.56-100 µg\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P48199","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10185MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10185MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10185MS",}