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小鼠高迁移率族蛋白B1(HMGB-1)酶联免疫吸附检测试剂盒

  • 货号:ELK10273
  • 价格:¥2,600.00 元
  • 点击:2498

产品介绍

产品信息

英文名称Mouse HMGB-1(High Mobility Group Protein B1) ELISA Kit
别名HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMG1; High Mobility Group Protein 1

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Mouse
检测类型Sandwich
灵敏度0.066 ng/mL
检测范围0.16-10 ng/mL
样本类型serum, plasma and other biological fluids
实验时长3.5h

研究信息

研究领域Infection immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P63158

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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