小鼠克拉拉细胞蛋白16(CC16)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Mouse CC16(Clara Cell Protein 16) Microsample ELISA Kit","alternative_names":"SCGB1A1; CC10; CCSP; UGB; Uteroglobin; Urinary Protein 1; Clara Cell Secretory Protein; Secretoglobin Family 1A Member 1; Clara-Cell Specific 10-kD Protein","species":"Mouse","assay_type":"Sandwich","sensitivity":"1.02 ng\/mL","standard":"200 ng\/mL","detection_range":"3.13-200 ng\/mL","sample_type":"serum, plasma, lung lavage fluid, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Infection immunity;Cardiovascular biology;Pulmonology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CC16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CC16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CC16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CC16 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10307MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10307MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10307MS",}