人胎儿血红蛋白(HBF)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human HBF(Fetal Hemoglobin) ELISA Kit","alternative_names":"hemoglobin F; Foetal Haemoglobin","species":"Human","assay_type":"Competitive Inhibition","sensitivity":"0.99 µg\/mL","standard":"200 µg\/mL","detection_range":"3.13-200 µg\/mL","sample_type":"serum, plasma, erythrocyte lysates and other biological fluids","assay_length":"2h","research_area":"Hematology;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBF in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10343-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10343-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10343",}